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產(chǎn)品名稱:Mouseanti-HumanIgG(Heavy&LightChain)Antibody
產(chǎn)品型號(hào):
產(chǎn)品廠商:半導(dǎo)體
產(chǎn)品文檔:
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簡(jiǎn)單介紹:
Mouseanti-HumanIgG(Heavy&LightChain)Antibody
詳情介紹:
Product details
Product details
Immunogen
Human IgG was isolated from human sera and purified by chromatography.
Clone
4D2D9G8-4A10
Isotype
IgG1
Characteristics
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Purification
Purified
Target details
Target details
Background
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molecular Weight
50 kDa
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Buffer
PBS containing 0.03 % sodium azide.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C or at -20 °C for long term.
Images
Images
Supplier Images
Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
×
Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4) .
Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4) .
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References
References
Product cited in:
Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture."
in:
Immunology
, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).