產(chǎn)品詳情

  • 產(chǎn)品名稱：HelaNuclearExtract

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：KamiyaBiomedical
  • 產(chǎn)品文檔：
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簡單介紹：
HelaNuclearExtract
詳情介紹：
Background Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
Application Notes Western blot: Use at 10-20 μg/lane HDAC. Fluorometric Assay: Use at 10 μg/assay HDAC. Colorometric Assay: Use at 50 μg/assay
Restrictions For Research Use only
Format Liquid
Buffer 200 μL of HeLa nuclear extract at 5 mg/mL in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl2, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF.
Storage -80 °C