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  • 產品名稱：GranzymeBInhibitor

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GranzymeBInhibitor
詳情介紹：
Purpose Caspase-6, 8, 9 Inhibitor?
Sequence Z-Ala-Ala-Asp(Ome)-CMK, Z-AAD-CMK
Characteristics Peptide-chloromethyl ketone inhibitor of Granzyme B. The CMK (choromethyl ketone) inhibitor has several advantages over other inhibitors.
Highly specific
Inhibits both human and murine Granzyme B
Irreversible inhibition
kobs/[I] (second-order inhibition constant) = 2.0 M-1s-1
For use on cell extracts only, not for use on live cells. Cell-mediated killing by cytotoxic T-lymphocytes (CTLs) is an important immunologic defense against tumor cell proliferation, viral infection, and transplanted tissue. Cell death induced by CTLs is mostly apoptotic and is thought to involve perforin, a pore-forming protein, and the granzymes, a family of serine proteases that are present in the cytoplasmic granules of CTLs and natural killer cells. Seven serine proteases (Granzymes A, B, C, D, E, F, and G) have been isolated from mouse CTL granules. Two serine proteases (Granzyme A and B) have been isolated from human CTL granules and are homologous to the two murine enzymes. Granzyme B is the granzyme most specifically found in CTLs and the granzyme shown to cause the most rapid kinetics of cell death. Granzyme B shares an unusual substrate specificity with interleuken-1beta converting enzyme (ICE), another enzyme involved in apoptosis, in that both require an Asp in the P1 position of the recognition sequence.
Purity 99.9%
Solubility DMSO
Molecular Weight 456 Da
Application Notes Highly specific inhibition of Granzyme B activity. For Granzyme B fluorometric assays using the Granzyme B Fluorogenic Substrate (Cat. No. AC-002), Granzyme B Inhibitor can be used to assess the contribution of contaminating proteases to the overall rate of proteolysis. For use on cell extracts only, not for use on live cells. The -CMK inhibitors are strong alkylating agents, and are very toxic.
Comment

Peptide-fluoromethyl ketone inhibitor of caspases.
The CH2F (fluoromethyl ketone) inhibitor has several advantages over other types of derivatives: Penetrates cell membranes, Not toxic to cells, Irreversible inhibition
Available data (reference 1) indicates that FMK inhibitors based on the sequence AEVD will inactivate caspases-6, 8, 9, and to a lesser extent caspases-1 and 3. Caspases-2, 4, 5, 7, and 10 will be little affected.
Dissolve the Caspase-13 Inhibitor in DMSO before use. It is important that dry, good quality DMSO be used.
Restrictions For Research Use only
Format Solid
Storage RT