產(chǎn)品詳情

  • 產(chǎn)品名稱：Z-IETD-FMK(Caspase-8Inhibitor)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：KamiyaBiomedical
  • 產(chǎn)品文檔：
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簡單介紹：
Z-IETD-FMK(Caspase-8Inhibitor)
詳情介紹：
Purpose Irreversible caspase-6, 8 inhibitor?
Sequence Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK
Specificity Highly specific inhibitor of caspase-8. May have some inhibitory activity towards caspase-6.
Characteristics Levels of DMSO above 0.2% may cause some cellular toxicity, thus masking the effect of the protease inhibitor. For extended use in vivo and in vitro: For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases. IMPORTANT NOTE for in vitro use: Our peptide inhibitors are synthesized as methyl esters to enhance cell permeability. In intact cells, the methyl groups are removed by endogenous enzymes. For in vitro experiments with purified enzymes, however, the methyl groups must first be removed by treating the inhibitor with esterase. A procedure is available upon request.
Chemical Name Z-IETD-fluoromethylketone
Formula C??H??FN?O??
Solubility DMSO
Molecular Weight 654 Da
Research Area Apoptosis/Necrosis, Cancer
Application Notes For caspase-8 fluorometric assays using the Caspase-8(FLICE) Fluorogenic Substrate, Ac- IETD-AFC, Caspase-8(FLICE) Inhibitor can be used to assess the contribution of contaminating proteases to the overall rate of proteolysis.
Comment

Peptide-fluoromethyl ketone inhibitor of caspase-8(FLICE). The CH2F or FMK (fluoromethyl ketone) inhibitor has several advantages over other types of derivatives: Penetrates cell membranes, is nontoxic to cells, irreversible inhibition.
Caspase-8 (also known as FLICE, MACH or Mch5) is a member of the caspase family of cysteine proteases. Caspases play an important role in apoptosis signaling and effector mechanisms. Caspase-8 is most similar to caspase-10, both of which have "death domain" motifs. Caspase-8 appears to be physically associated with the signaling mechanism during Fas-mediated cell death and its association with Fas or tumor necrosis factor receptors via an interaction with FADD suggests it functions as an initiator rather than an effector of the cell death pathway. Thus, caspase-8 is an upstream activator in the protease cascade that proteolytically matures other caspases.
Highly specific inhibitor of caspase-8. May have some inhibitory activity towards caspase-6.
For caspase-8 fluorometric assays using the Caspase-8(FLICE) Fluorogenic Substrate, AcIETD-AFC (ABIN924941), Caspase-8(FLICE) Inhibitor can be used to assess the contribution of contaminating proteases to the overall rate of proteolysis.
Dissolve the Caspase-8(FLICE) Inhibitor in high purity (>99.9%) DMSO before use.

For use on intact cells:
1. Prepare desired concentrated stock solutions as follows:
3 mg Z-IETD-FMK
in 45μl DMSO = 10 mM

2. Adding 2 μL of a 10 mM stock solution to 1 mL of culture medium gives a final Z-IETD- FMK concentration of 20 μM. Effective final concentrations are estimated to be 5-20 μM.
Restrictions For Research Use only
Format Solid
Storage RT