產(chǎn)品詳情

  • 產(chǎn)品名稱：LDHD ELISA Kit(LactateDehydrogenaseD)

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  • 產(chǎn)品廠商：國內(nèi)供應3
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LDHD ELISA Kit(LactateDehydrogenaseD)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.
Sample Type Serum, Tissue Homogenate, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural Mouse D-LDH.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4
Alternative Name Ldhd (LDHD ELISA Kit Abstract)
Pathways Ribonucleoside Biosynthetic Process
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.