產(chǎn)品詳情

  • 產(chǎn)品名稱：IL17A ELISA Kit(Interleukin17A)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
IL17A ELISA Kit(Interleukin17A)
詳情介紹：
Purpose This immunoassay kit allows for the specific measurement of mouse IL-17 concentrations in cell culture supernates, serum, and plasma.
Sample Type Cell Culture Supernatant, Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IL-17.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Characteristics Mus musculus,Mouse,Interleukin-17A,IL-17,IL-17A,Cytotoxic T-lymphocyte-associated antigen 8,CTLA-8,Il17a,Ctla8,Il17
Alternative Name Il17a (IL17A ELISA Kit Abstract)
Background Interleukin 17 (IL-17, also known as IL-17A) is a 30 - 35 kDa variably glycosylated homodimeric protein that belongs to a unique family of cysteine-knot related proteins. Its sequence was originally isolated from an activated rodent hybridoma and termed CTLA-8 . It is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 15 kDa, 132 aa mature segment. Although there are two intrachain disulfide bonds that create a ring reminiscent of those found in cysteine-knot proteins, the actual closed knot structure does not appear to form. IL-17 has one potential N-linked glycosylation site. Mature rat IL-17 is 61% and 60% aa identical to mouse and rat IL-17, respectively. It also shows limited aa sequence identity to other rat IL-17 family members. In particular, it shows 34%, 38%, 34% and 26%aa sequence identity to IL-17B, C, D, and E, respectively, and 49% aa sequence identity to IL-17F with which it is most homologous. While rodent and rat mature sequences show modest aa sequence identity, rat IL-17 is active on both mouse and rat cells. The cells principally known to produce IL-17 are the memory CD4+ T cells. In addition, CD8+ T cells as well as TCR+ CD4-CD8- T cells, neutrophils (PMNs) and eosinophils have also been reported to express mRNA transcripts for IL-17.
Sample Volume 100 μL
Plate Pre-coated
Protocol This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-17 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.