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  • 產(chǎn)品名稱：PTGS1 ELISA Kit(Prostaglandin-EndoperoxideSynthase1(ProstaglandinG/HSynthaseandCy

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  • 產(chǎn)品廠商：國內(nèi)供應3
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簡單介紹：
PTGS1 ELISA Kit(Prostaglandin-EndoperoxideSynthase1(ProstaglandinG/HSynthaseandCyclooxygenase))
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse SDF-1? concentrations in serum, plasma and other biological fluids.
Sample Type Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse SDF-1?.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Prostaglandin G/H synthase 1,Cyclooxygenase-1,COX-1,Prostaglandin H2 synthase 1,PGH synthase 1,PGHS-1,PHS 1,Prostaglandin-endoperoxide synthase 1,Ptgs1,Cox-1,Cox1,1.14.99.1
Alternative Name Ptgs1 (PTGS1 ELISA Kit Abstract)
Background SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and is therefore an important haematopoietic growth factor for these. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow).
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1β. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SDF-1β. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SDF-1β, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of SDF-1β in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.