產(chǎn)品詳情

  • 產(chǎn)品名稱：Fibronectin ELISA Kit(Fibronectin1)

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  • 產(chǎn)品廠商：國(guó)內(nèi)供應(yīng)3
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Fibronectin ELISA Kit(Fibronectin1)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse Fibronectin,FN concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse FN.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 11.5 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Fibronectin,FN,Fn1
Alternative Name Fn1 (FN1 ELISA Kit Abstract)
Pathways Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.