產(chǎn)品詳情

  • 產(chǎn)品名稱：serpinPeptidaseInhibitor,CladeF(Alpha-2Antiplasmin,PigmentEpitheliumDerivedFact

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
  • 產(chǎn)品文檔：
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serpinPeptidaseInhibitor,CladeF(Alpha-2Antiplasmin,PigmentEpitheliumDerivedFactor),Member1(SERPINF1) ELISA Kit
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse PEDF concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse PEDF.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 0.39 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Pigment epithelium-derived factor,PEDF,Caspin,Serpin F1,Stromal cell-derived factor 3,SDF-3,Serpinf1,Pedf,Sdf3
Alternative Name Serpinf1 (SERPINF1 ELISA Kit Abstract)
Background Pigment epithelium-derived factor (PEDF) is a multipotent neurotrophic factor with potent activity both in the central nervous system and in the retina. To date, PEDF is the most potent angiogenic inhibitor found in the mammalian ocular compartment. Interestingly, recent studies have shown that decreased ocular levels of PEDF are associated with diabetic retinopathy and other neuroretinal dystrophic diseases. In addition, the neurotropic and anti-angiogenic properties of PEDF suggest this protein is of potential therapeutic value for control of neovascularization associated with tumor growth.
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEDF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PEDF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PEDF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PEDF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.