產(chǎn)品詳情

  • 產(chǎn)品名稱：Aquaporin4 ELISA Kit(AQP4)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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Aquaporin4 ELISA Kit(AQP4)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse AQP-4 concentrations in tissue homogenates and other biological fluids.
Sample Type Tissue Homogenate, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse AQP-4.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Aquaporin-4,AQP-4,Mercurial-insensitive water channel,MIWC,WCH4,Aqp4
Alternative Name Aqp4 (AQP4 ELISA Kit Abstract)
Background Aquaporin-4 (AQP4), is a channel protein that allows water to move in and out of cells. Aquaporin-4 is located in many tissues, including the basolateral membrane of the renal cortical and medullary principal cell, as well as in the brain.Aquaporin-4 is the first specific molecule to be defined as a target for the autoimmune response in any form of multiple sclerosis. Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.
Pathways Sensory Perception of Sound
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of AQP-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.