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  • 產(chǎn)品名稱:Aquaporin1 ELISA Kit(Aquaporin1(ColtonBloodGroup))

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Aquaporin1 ELISA Kit(Aquaporin1(ColtonBloodGroup))
詳情介紹:
Purpose This immunoassay kit allows for the specific measurement of human AQP1 concentrations in cell culture supernates, tissue homogenates serum, and plasma.
Sample Type Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural human AQP1.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 0.04 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Homo sapiens,Human,Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28
Alternative Name AQP1 (AQP1 ELISA Kit Abstract)
Background AQP1 is a polypeptide hormone produced in pancreatic beta-cells that belongs to the family of calcitonin gene-related peptides. There is a 20% sequence homology between AQP1 and calcitonin and 44% homology with calcitonin gene-related peptide. AQP1 and its fragments stimulate the proliferation of osteoblasts, inhibit bone resorption, and increase bone density and the amount of bone mass.
Pathways Hormone Transport
Sample Volume 100 μL
Plate Pre-coated
Protocol This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for AQP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AQP1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for AQP1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.