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  • 產(chǎn)品名稱：IL18 ELISA Kit(Interleukin18)

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IL18 ELISA Kit(Interleukin18)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 18,IL-18 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IL-18.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Interleukin-18,IL-18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Il18,Igif
Alternative Name Il18 (IL18 ELISA Kit Abstract)
Background Interleukin 18 (IL-18) is a 18 kDa novel cytokine which is identified as a costimulatory factor for production of interferon- γ (IFN- γ ) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 ? converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 acts on T helper 1-type T (Th1) cells and in combination with IL-12 strongly induces production of IFN- γ by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN- γ and GM-CSF in peripheral blood mononuclear cells, production of T helper type 1 cytokines, IL-2, GM-CSF and IFN- γ in T cells, enhancement of Fas ligand expression by T helper type 1 cells.
Pathways Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.