產(chǎn)品詳情

  • 產(chǎn)品名稱：IFNG ELISA Kit(Interferongamma)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
IFNG ELISA Kit(Interferongamma)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse IFN- ? concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IFN- ? .
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Interferon gamma,IFN-gamma,Ifng
Alternative Name Ifng (IFNG ELISA Kit Abstract)
Background IFN- γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor. The IFN- γ monomer consists of a core of six α -helices and an extended unfolded sequence in the C-terminal region. The biologically active dimer is formed by anti-parallel inter-locking of two monomers. Full length of IFN- γ is 143 amino acids. In contrast to interferon- α and interferon- β which can be expressed by all cells, IFN- γ is secreted by T lymphocytes and NK cells only. Also known as immune interferon, IFN- γ is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN- γ has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by IFN- γ is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN- γ binding to the receptor activates the JAK-STAT pathway. In addition, IFN- γ activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN- γ is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and [[CD8+ cytotoxic T cell]]s also produce IFN- γ . IFN- γ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF κ B.
Pathways Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN- γ . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFN- γ . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFN- γ , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a 2 change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFN- γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.