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  • 產(chǎn)品名稱：IL5 ELISA Kit(Interleukin5)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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IL5 ELISA Kit(Interleukin5)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of human Interleukin 5,IL-5 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural human IL-5.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Homo sapiens,Human,Interleukin-5,IL-5,B-cell differentiation factor I,Eosinophil differentiation factor,T-cell replacing factor,TRF,IL5
Alternative Name IL5 (IL5 ELISA Kit Abstract)
Background Il-5 is a 135 amino acid (aa) polypeptide with a predicted mass of 12.5 kDa. It is secreted by a restricted number of mesenchymal cell types. In its native state, mature IL-5 is synthesized as a 115 aa, highly glycosylated 22 kDa monomer that forms a 40-50 kDa disulfide-linked homodimer. Although the content of carbohydrate is high, carbohydrate is not needed for bioactivity. Monomeric IL-5 has no activity and requires a homodimer for function, which is in contrast to the receptor-related IL-3 and GM-CSF cytokines that exist only as monomers. Just as one IL-3 and GM-CSF monomer binds to one receptor, one IL-5 homodimer is able to engage only one IL-5 receptor. It has been suggested that IL-5 (as a dimmer) undergoes a general conformational change after binding to one receptor molecule and this change precludes binding to a second receptor. Mouse and mouse mature IL-5 are 71% identical at the aa level. IL-5 appears to perform a number of functions on eosinophils including growth and differentiation, the down modulation of Mac-1, the upregulation of receptors for IgA and IgG, the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release. IL-5 may act in an adjunct fashion plays, however, the exact role is unclear. Finally, there is a great deal of interest in the interaction between IL-5 and the CC chemokine eotaxin. Studies suggest that inflammatory-induced and locally produced IL-5 and eotaxin may act on the bone marrow in a cooperative manner.
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution 2 and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.