產品詳情

  • 產品名稱：HeLaNuclearExtract

  • 產品型號：
  • 產品廠商：Biovision
  • 產品文檔：
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簡單介紹：
HeLaNuclearExtract
詳情介紹：
Characteristics Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
Application Notes Western blot: 10-20 μg/lane HDAC Fluorometric Assay: 10 μg/assay HDAC Colorimetric Assay: 50 μg/assay
Restrictions For Research Use only
Concentration 5 mg/mL
Storage -80 °C
Expiry Date 12 months
Background publications Dignam, Lebovitz, Roeder: "Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei." in: Nucleic acids research, Vol. 11, Issue 5, pp. 1475-89, 1983 (PubMed).