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  • 產品名稱：Caspase-13SubstrateLEED-AFC

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  • 產品廠商：Biovision
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Caspase-13SubstrateLEED-AFC
詳情介紹：
Sequence Ac-Leu-Glu-Glu-Asp-AFC
Characteristics Ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Purity > 98?% by HPLC
Chemical Name Ac-LEED-AFC, Caspase-13 Substrate, Fluorogenic
Formula C??H??F?N?O??
Permeability Not-permeable
Molecular Weight 757.66 g/mol
Restrictions For Research Use only
Format Liquid
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Product cited in: Baumgartner, Gerasimenko, Thorne, Ashurst, Barrow, Chvanov, Gillies, Criddle, Tepikin, Petersen, Sutton, Watson, Gerasimenko: "Caspase-8-mediated apoptosis induced by oxidative stress is independent of the intrinsic pathway and dependent on cathepsins." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 293, Issue 1, pp. G296-307, 2007 (PubMed).