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  • 產品名稱：CaspaseFluorometricSubstrateSetPlus

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CaspaseFluorometricSubstrateSetPlus
詳情介紹：
Purpose Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
Sample Type Cell Culture Cells
Detection Method Fluorometric
Characteristics Caspase-Family Fluorometric Substrate Set Plus: Ready-to-Use Fluorometric substrates for assaying activities of caspase-1, -2, -3, -5, -6, -8 & -9 family proteases. Concentration: 1mM. Also includes Cell lysis Buffer, 2X Reaction Buffer & DTT.
Components Caspase-1 Substrate, Ac-YVAD-AFC
Caspase-2 Substrate, Ac-VDVAD-AFC
Caspase-3 Substrate, Ac-DEVD-AFC
Caspase-5 Substrate, Ac-WEHD-AFC
Caspase-6 Substrate, Ac-VEID-AFC
Caspase-8 Substrate, Ac-IETD-AFC
Caspase-9 Substrate, Ac-LEHD-AFC
Cell Lysis Buffer
2X Reaction Buffer
DTT (1 M)
Alternative Name Caspase
Background Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
Application Notes Detects specific Caspses involved in apoptosis within cells
Comment

Further details regarding sample type: Cell culture
Protocol 1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5 x 10^6 cells or use 50-200 μg cell lysates if protein concentration has been measured.
3. Resuspend cells in 50 μL of chilled Cell Lysis Buffer.
4. Incubate cells on ice for 10 minutes.
5. Add 50 μL of 2X Reaction Buffer and 1 μL DTT to each sample.
6. Add 5 μL of the 1 mM AFC conjugated substrates (50 μ M final conc.) into each tube individually and incubate at 37 °C for 1-2 hour.
7. Read samples in a fluorometer equipped with a 400-nm excitation filter and 505-nm emission filter. For a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay directly in a 96-well plate. Fold-increase in caspase activity can be determined by comparing these results with the level of the uninduced control.
Restrictions For Research Use only
Concentration 1 mM
Storage -20 °C
Expiry Date 6-12 months