產(chǎn)品詳情

  • 產(chǎn)品名稱：Caspase-2SubstrateVDVAD-pNA

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  • 產(chǎn)品廠商：Biovision
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簡單介紹：
Caspase-2SubstrateVDVAD-pNA
詳情介紹：
Sequence Ac-Val-Asp-Val-Ala-Asp-pNA
Characteristics Ready-to-use colorimetric substrate for caspase-2/Ich-1 and related caspases that recognize the amino acid sequence VDVAD. Caspase-2 and related caspase activity can be quantified by detection of free pNA after cleavage from the peptide substrate VDVAD-pNA at OD405 nm using a spectrophotometer or plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays.
Purity > 98?% by HPLC
Chemical Name Ac-VDVAD-pNA, Caspase-2 Substrate, Colorimetric, VDVAD-pNA
Formula C??H??N?O??
Permeability Not-permeable
Molecular Weight 679.7 g/mol
Comment

Note: Background reading from cell lysates and buffers should be subtracted from the readings of both induced and the uninduced samples before calculating fold increase in caspase activity.
Protocol 1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5 x 10^6 cells.
3. Resuspend cells in 50 μL of chilled Cell Lysis Buffer and incubate cells on ice for 10 minutes.
4. Centrifuge for 1 min in a microcentrifuge (10,000 x g).
5. Transfer supernatant (cytosolic extract) to a fresh tube and put on ice.
6. Assay protein concentration.
7. Dilute 50-200 μg protein to 50 μL Cell Lysis Buffer for each assay.
8. Add 50 μL of 2X Reaction Buffer containing 10 mM DTT to each sample.
9. Add 5 μL of the 4 mM of VDVAD-pNA (200 μ M final conc.) and incubate at 37 °C for 1-2 hour.
9. Read samples at 400- or 405-nm in a microtiter plate reader, or spectrophotometer using a 100- μL micro quartz cuvet (Sigma), or dilute sample to 1 mL with Dilution Buffer and using regular cuvet (note: Dilution of the samples proportionally decreases the reading). You may also perform the entire assay directly in a 96-well plate. Fold-increase in caspase activity can be determined by comparing these results with the level of the uninduced control. Note: Background reading from cell lysates and buffers should be subtracted from the readings of both induced and the uninduced samples before calculating fold increase in caspase activity.
Restrictions For Research Use only
Format Liquid
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Product cited in: Basu, Adkins, Basu: "Down-regulation of caspase-2 by rottlerin via protein kinase C-delta-independent pathway." in: Cancer research, Vol. 68, Issue 8, pp. 2795-802, 2008 (PubMed).

Lee, Cherla, Tesh: "Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1." in: Infection and immunity, Vol. 75, Issue 3, pp. 1291-302, 2007 (PubMed).

Doostzadeh-Cizeron, Yin, Goodrich: "Apoptosis induced by the nuclear death domain protein p84N5 is associated with caspase-6 and NF-kappa B activation." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25336-41, 2000 (PubMed).