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  • 產(chǎn)品名稱：CaspaseFluorometricSubstrateSetII

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  • 產(chǎn)品廠商：Biovision
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CaspaseFluorometricSubstrateSetII
詳情介紹：
Purpose Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
Sample Type Cell Culture Cells
Detection Method Fluorometric
Characteristics Caspase-Family Fluorometric Substrate Set II: Ready-to-Use Fluorometric Substrates for Assaying Activities of Caspase-1, -2, -3, -4, -5, -6, -8, -9 & -10 family proteases. Concentration: 1 mM.
Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form. Kit CONTENTS: 125 μL (1 mM) each of the following 9 substrates dissolved in DMSO: Caspase-1 Substrate, Ac-YVAD-AFC Caspase-2 Substrate, Ac-VDVAD-AFC Caspase-3 Substrate, Ac-DEVD-AFC Caspase-4 Substrate, Ac-LEVD-AFC Caspase-5 Substrate, Ac-WEHD-AFC Caspase-6 Substrate, Ac-VEID-AFC Caspase-8 Substrate, Ac-IETD-AFC Caspase-9 Substrate, Ac-LEHD-AFC Caspase-10 Substrate, Ac-AEVD-AFC
Components Caspase-1 Substrate, Ac-YVAD-AFC
Caspase-2 Substrate, Ac-VDVAD-AFC
Caspase-3 Substrate, Ac-DEVD-AFC
Caspase-4 Substrate, Ac-LEVD-AFC
Caspase-5 Substrate, Ac-WEHD-AFC
Caspase-6 Substrate, Ac-VEID-AFC
Caspase-8 Substrate, Ac-IETD-AFC
Caspase-9 Substrate, Ac-LEHD-AFC
Caspase-10 Substrate, Ac-AEVD-AFC
Alternative Name Caspase
Background Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
Application Notes Detects specific Caspses involved in apoptosis within cells
Comment

Further details regarding sample type: Cell culture
Protocol 1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5 x 10^6 cells or use 50-200 μg cell lysates if protein concentration has been measured.
3. Resuspend cells in 50 μL of chilled Cell Lysis Buffer containing 10 mM DTT to each sample.
6. Add 5 μL of the 1 mM AFC conjugated substrates (50 μ M final conc.) into each tube individually and incubate at 37 °C for 1-2 hour.
7. Read samples in a fluorometer equipped with a 400-nm excitation filter and 505-nm emission filter. For a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay directly in a 96-well plate. Fold-increase in caspase activity can be determined by comparing these results with the level of the uninduced control.
Restrictions For Research Use only
Concentration 1 mM
Storage -20 °C
Expiry Date 6-12 months