產(chǎn)品詳情

  • 產(chǎn)品名稱：AgaAGeneProduct(AGAA)protein

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Biovision
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
AgaAGeneProduct(AGAA)protein
詳情介紹：
Purpose Pure recombinant bacterial agarase
Background Agarase cleaves agarose to neoagaro-oligosaccharides, and is used for quantitative, gentle recovery of DNA from low melting point agarose.
Alternate Names: Agarase
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Unit Definition: One unit is defined as the amount of enzyme required to digest 100 μl (approx. 100 mg) of molten 1% low melting point agarose to neoagaro-oligosaccharides in 1 hour at 45°C.
Protocol Note: When the percentage of agarose in higher than 1 %, the units of agarase have to be adjusted in proportion. When using Tris-borate gel electrophoresis buffer, a two-fold amount of agarase should be used, otherwise, the incubation time has to be prolonged.
4. Carefully mix the solution and incubate for 1 hour at 42-45 °C.
5. Add 0.1 volume of 3 M sodium acetate, pH 5.5, to the melten agarose solution and incubate 15 min on ice.
6. Centrifuge for 15 minutes at 2-8 °C to pellet the oligosacchrides.
7. Precipitate the nucleic acids from the supernatant with 3 volume of ice-cold ethanol as usual.
Restrictions For Research Use only
Format Liquid
Concentration 2.0 units/μL
Buffer 40 mM Tris-HCl,?pH 7.5, 50 mM NaCl, 50?% glycerol (v/v).
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months