產(chǎn)品詳情

  • 產(chǎn)品名稱：RIPALysisBuffer(Strong)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Biospes
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簡單介紹：
RIPALysisBuffer(Strong)
詳情介紹：
Characteristics 1. This RIPA Lysis Buffer is a kind of traditional cell and tissue lysis buffer. The end protein / sample lysed by this buffer can be used for Western Blot and Immunoprecipitation. 2. RIPA (Radio Immunoprecipitation Assay) Lysis Buffer has different formulas, and according to its lysis strength, it can be roughly classified into three categories: strong, medium and weak. 3. RIPA Lysis Buffer (Medium) contains: 50mM Tris (pH 7.4), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, and different kinds of inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin), which can effectively inhibit the protein degradation. 4. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit. Kit Components Components
Sample Preparation

Cultured Cells Sample 1. Pipette proper volume of RIPA Lysis Buffer and mix thoroughly. 2-3 min advanced before use, add PMSF buffer to make its final concentration to1mM. 2. For adherent cells: wash sample with PBS, normal saline or serum-free culture medium to remove culture solution. Add proper volume of RIPA Lysis Buffer, then stroke with pipette until the buffer immerse cells completely. Shake slightly for 5-10 min. After lysis, centrifuge at 10,000-14,000 g for 10 min, then collect the supernatants and move on to the next step. >> Instruction for RIPA usage: for different sizes of cell culture plates <<
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment RIPA Lysis Buffer (Medium) 100 ml Store at 4 °C for one year