產(chǎn)品詳情

  • 產(chǎn)品名稱：CellLysisBufferforWB&IP

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Biospes
  • 產(chǎn)品文檔：
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CellLysisBufferforWB&IP
詳情介紹：
Characteristics 1. Cell Lysis Buffer for Western and IP is a kind of lysis buffer which lyses cells under non-denatured condition. The end cells lysed by this buffer can be used for PAGE, Western Blot, Immunoprecipitation and Co-Immunoprecipitation. 2. This product contains: 20mM Tris (pH 7.5), 150mM NaCl, 1% Triton X-100 and different kinds of inhibitors (sodium pyrophosphate, beta-glycerophosphate, EDTA, Na 3 VO 4 , leupeptin), which can effectively inhibit the protein degradation and maintain the original protein interaction. 3. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit. Kit Components Components
Sample Preparation

Cultured Cells Sample 1. Pipette proper volume of the lysis buffer and mix thoroughly. 2-3 min advanced before use, add 2. buffer to make its final concentration to1mM. 2. For adherent cells: wash sample with PBS, normal saline or serum-free culture medium to remove culture solution. Add proper volume of the lysis buffer, then stroke with pipette until the buffer immerse cells completely. Shake slightly for 5-10 min. After lysis, centrifuge at 10,000-14,000 g for 10 min, then collect the supernatants and move on to the next step. >> Instruction for the buffer usage: for different sizes of cell culture plates <<
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cell Lysis Buffer for WB & IP 100 ml Store at -20 °C for one year
Expiry Date 12 months