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產(chǎn)品名稱:SDS-PAGESampleLoadingBuffer(6X)
產(chǎn)品型號:
產(chǎn)品廠商:Biospes
產(chǎn)品文檔:
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簡單介紹:
SDS-PAGESampleLoadingBuffer(6X)
詳情介紹:
Product details
Product details
Characteristics
SDS-PAGE Sample Loading Buffer is a kind of 6 × concentrated solution which based on Bromophenol blue dye. This reagent contains SDS, DTT, Glycerol, Bromophenol blue, Tris-HCL buffer, ect. It can be used for common SDS-PAGE protein sample loading. Kit Components Components
Application Details
Application Details
Sample Preparation
Dissolve the SDS-PAGE Sample Loading Buffer, 6 × in water bath at room temperature or not exceeding 37℃. After dissolving, store at room temperature immediately. 2. Mix sample with SDS-PAGE Sample Loading Buffer, 6 × at the ratio 5:1 (i.e. add 5myl sample into 1myl of SDS-PAGE Sample Loading Buffer). 3. Fully denaturize sample at 100℃ or boiling water bath for 3-5 min. 4. Cool the sample to room temperature, then load and electrophorese. 5. Stop electrophoresis when Bromophenol blue dye arrives at the bottom of gels.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Preservative
Sodium azide, Thimerosal (Merthiolate)
Precaution of Use
This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
SDS-PAGE Sample Loading Buffer, 6 × 1 ml × 5 Store at -20°C at least for one year.