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Sample Preparation
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- After electrophoresis, wash gel with distilled water by agitating for 3 times, 5 min each to remove the interferent, like salt, SDS, etc. 2. Remove the distilled water, add small volume of Coomassie Brilliant Blue Fast Staining Solution (agitate before use) to immerse the gel. 3. According to the protein quantity, the band for large quantity of protein will appear within 10-30 min, but the majority bands will appear within 1-2 hours. To obtain more sensitive band or to speed up the staining, end user can immerse the gel in Coomassie Brilliant Blue Fast Staining Solution, and water bath at 95-100°C or heat by a baking or microwave oven to 95-100°C, then stain on a shaker. When the staining solution cool to room temperature, water bath or heat to 95-100°C and stain on a shaker, repeat this for 2-3 times. Note: Avoid boiling when heat to prevent the gel break. 4. Stop staining when the target bands appear obviously. Remove the staining solution, add appropriate volume of distilled water to stop the staining reaction. Then, photograph and record the results. Note: To obtain more clear bands, wash the gel with RT distilled water or immerse in 4°C distilled water overnight. Or after immersing in 4°C distilled water overnight, the next day, stain and wash the stained gel in the same way, the staining efficiency can be improved, and can get better bands. Chongqing Biospes Co., Ltd Product Manual FOR RESEARCH USE ONLY, NOT FOR DIAGNOSTIC AND CLINICAL USE. Chongqing Biospes Co., Ltd Tel: +86-23-67567091 Fax: +86-23-67745923 Room 27-2, Building 7, No. 15, Paradise Walk, Jiangbei District, Chongqing, 400020, China www.biospes.com 2 Trouble Shooting 1. No bands appear: the loading volume may be small, it is recommended to set two BSA lanes with different volumes as positive control during electrophoresis. 2. High background: the interferent may not remove thoroughly, it is recommended to increase wash after electrophoresis. 3. If precipitate appears when stain the gel, the container may be contaminated. It is recommended to change a clean container, and add the fresh staining solution to continue staining until get obvious bands.
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