產(chǎn)品詳情
  • 產(chǎn)品名稱:LlamaIgGisotypecontrol(FITC)

  • 產(chǎn)品型號:
  • 產(chǎn)品廠商:BioLeaf
  • 產(chǎn)品文檔:
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簡單介紹:
LlamaIgGisotypecontrol(FITC)
詳情介紹:
Isotype IgG
Purification Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain), non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa, non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.
ProductDetails: Labeling Ratio 3.2
Research Area Immunology, Secondary Antibodies
Application Notes

Flow Cytometry Dilution: User Optimized

Application Note: Llama IgG Fluorescein can be utilized as a control or standard reagent in SDS, Western Blotting, Flow Cytometry, Immunofluorescence, and FLISA experiments. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2

Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months