產(chǎn)品詳情

  • 產(chǎn)品名稱：RatIgG1IsotypeControl

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡單介紹：
RatIgG1IsotypeControl
詳情介紹：
Clone RG1
Isotype IgG1
Purification RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG2a, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Research Area Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:1000-1:5000

Application Note: RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized
Comment

RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride,?pH 7.2

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months