產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：HumanIgGisotypecontrol(APC)

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  • 產(chǎn)品廠(chǎng)商：BioLeaf
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HumanIgGisotypecontrol(APC)
詳情介紹：
Isotype IgG
Cross-Reactivity (Details) Human IgG whole molecule Allophycocyanin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Allophycocyanin, anti-Human IgG and anti-Human Serum.
Purification Human IgG whole molecule Allophycocyanin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
ProductDetails: Labeling Ratio 4-6
Research Area Immunology, Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:500-1:2500

Immunohistochemistry Dilution: 1:500-1:2500

Application Note: Human IgG whole molecule Allophycocyanin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Western Blot Dilution: 1:1,000-1:5,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0?mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2

Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months