產品詳情

  • 產品名稱：HumanIgGisotypecontrol(PE)

  • 產品型號：
  • 產品廠商：BioLeaf
  • 產品文檔：
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簡單介紹：
HumanIgGisotypecontrol(PE)
詳情介紹：
Isotype IgG
Cross-Reactivity (Details) Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.
Purification Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
ProductDetails: Labeling Ratio 1-2
Research Area Immunology, Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:500-1:2500

Immunohistochemistry Dilution: 1:500-1:2500

Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Western Blot Dilution: 1:1,000-1:5,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0?mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2

Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Expiry Date 12 months