產(chǎn)品詳情

  • 產(chǎn)品名稱：MouseIgG2bisotypecontrol(PE-Cy5)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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MouseIgG2bisotypecontrol(PE-Cy5)
詳情介紹：
Isotype IgG2b
Characteristics This antibody can be used as a mouse IgG2b isotype control in flow cytometry and other applications.
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Assay Procedure
  • Take 100?μL peripheral blood anticoagulated by EDTA and add to the bottom of 5?mLtube,
  • Add 20?μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20?minutes at room temperature away from light,
  • Add 2 ml1×RBC lysis buffer, incubate for 10?minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5?minutes, discard the supernatant,
  • Add 2?mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5?minutes, discard the supernatant,
  • Add 0.5?mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4?°C then measured).
  • [PBS wash buffer: PBS +1?% FBS +0.1?% NaN3, Cat.: FXP005]
  • [Cell fixation: 2?% formaldehyde solution]
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered solution,?pH 7.4, containing and 0.2?% (w/v) BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Flow Cytometry (FACS) image for Mouse IgG2b isotype control (PE-Cy5) (ABIN2145356) Mouse IgG2b isotype control (PE-Cy5)