產(chǎn)品詳情

  • 產(chǎn)品名稱：MouseIgMIsotypeControl

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
MouseIgMIsotypeControl
詳情介紹：
Isotype IgM
Characteristics This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
Purification Purified
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Assay Procedure
  • Take 100?μL peripheral blood anticoagulated by EDTA and add to the bottom of 5?mLtube,
  • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30?minutes at room temperature,
  • Add 2 ml1×RBC lysis buffer, incubate for 10?minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5?minutes, discard the supernatant,
  • Add 2?mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5?minutes, discard the supernatant,
  • Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20?minutes away from light at room temperature.
  • Repeat step 5.
  • Add 0.5?mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4?°C then measured).
  • [PBS wash buffer: PBS +1?% FBS +0.1?% NaN3, Cat.: FXP005]
  • [Cell fixation: 2?% formaldehyde solution]
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered solution,?pH 7.4,
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Flow Cytometry (FACS) image for Mouse IgM isotype control (ABIN2145425) Mouse IgM isotype control