產(chǎn)品詳情

  • 產(chǎn)品名稱：LlamaIgG1isotypecontrol(HRP)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡單介紹：
LlamaIgG1isotypecontrol(HRP)
詳情介紹：
Isotype IgG1
Purification Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to llama IgG2 or IgG3 are observed.
Research Area Secondary Antibodies
Application Notes

Flow Cytometry Dilution: User Optimized

Application Note: Llama IgG1 HRP Conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized
Comment

Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35?% - 70?% of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses. All gamma chain camelid antibodies exhibiting the more conventional assembly of two light and two heavy chains with molecular weight ~150 kDa, belong to the IgG1 subclass.
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2

Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months