產(chǎn)品詳情

  • 產(chǎn)品名稱：LlamaIgG2isotypecontrol(FITC)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
LlamaIgG2isotypecontrol(FITC)
詳情介紹：
Isotype IgG
Purification Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
Alternative Name IgG
Research Area Secondary Antibodies
Application Notes

Flow Cytometry Dilution: User Optimized

Application Note: Llama IgG2 FITC conjugated isotype control can be utilized in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized
Comment

Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2

Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months