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Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG2 Biotin conjugated isotype control can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2
Stabilizer: 10?mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free