產(chǎn)品詳情

  • 產(chǎn)品名稱：RatImmunoglobulinG2a(IgG2a)isotypecontrol(FITC)

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  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡單介紹：
RatImmunoglobulinG2a(IgG2a)isotypecontrol(FITC)
詳情介紹：
Isotype IgG2a
Purification RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
ProductDetails: Labeling Ratio 2-8
Alternative Name IgG2a
Research Area Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:1000-1:5000

Application Note: RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

FLISA Dilution: User Optimized

ELISA Dilution: 1:2000-1:20,000

IF Microscopy Dilution: User Optimized
Comment

IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
Expiry Date 12 months