產(chǎn)品詳情

  • 產(chǎn)品名稱：MANF293CellLysate

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：Abbiotec
  • 產(chǎn)品文檔：
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MANF293CellLysate
詳情介紹：
Characteristics Antigen standard for mesencephalic astrocyte-derived neurotrophic factor (MANF) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC221555, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name MANF
Background Synonyms: arginine-rich protein, arginine-rich, mutated in early stage tumors, MANF, ARMET, ARP, MGC142148, MGC142150, Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutated in early stage tumor
NCBI Accession NP_006001
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for MANF 293 Cell Lysate (ABIN1595759) MANF lysate validation: The MANF 293 Cell Lysate (lane B) and Control Vector Cell Lys...