產品詳情

  • 產品名稱：PC-12nuclearextract(Forskolinstimulated)

  • 產品型號：
  • 產品廠商：ActiveMotif
  • 產品文檔：
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PC-12nuclearextract(Forskolinstimulated)
詳情介紹：
Characteristics

Origin: Rat adrenal gland, pheochromocytoma (kidney tumor)
Components 2 x 100 μg of PC-12 nuclear extract (Forskolin stimulated) at 2.50 μg/μl
Restrictions For Research Use only
Concentration 2.50 μg/μL
Buffer The PC12 nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with forskolin (20 μM). The Lysis Buffer consists of 20 mM Hepes?pH 7.5, 400 mM NaCl, 20?% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na2MoO4, 1 mM NaVO3, 10?mM PNPP, 10 mM b-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
Storage -80 °C
Storage Comment To ensure stability, extracts should be stored at -80°C.