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  • 產(chǎn)品名稱：Ratanti-Mouse(Murine)ImmunoglobulinG2a(IgG2a)(HeavyChain),(Chaingamma)Antibody(

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  • 產(chǎn)品廠商：Acris-antibodies
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Ratanti-Mouse(Murine)ImmunoglobulinG2a(IgG2a)(HeavyChain),(Chaingamma)Antibody(FITC)
詳情介紹：
Immunogen Spleen cells from immunised LOU/c rats were fused with cells of the IR983F myeloma cell line.
Clone LO-MG2a-7
Isotype IgG1
Purification Affinity Chromatography
Alternative Name IgG2a
Background Synonyms: Mouse Immunoglobulin 2a
Research Area Secondary Antibodies
Application Notes Flow cytometry: 5.0Mg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09?% Sodium Azide, 50?% Glycerol
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Jarman, Lamb: "Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling." in: Immunology, Vol. 112, Issue 4, pp. 631-42, 2004 (PubMed).

Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum." in: Journal of immunological methods, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).

Bazin, Xhurdebise, Burtonboy, Lebacq, De Clercq, Cormont: "Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants." in: Journal of immunological methods, Vol. 66, Issue 2, pp. 261-9, 1984 (PubMed).

Bazin, Beckers, Urbain-Vansanten, Pauwels, Bruyns, Tilkin, Platteau, Urbain: "Transplantable IgD immunoglobulin-secreting tumors in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 121, Issue 5, pp. 2077-82, 1979 (PubMed).

Querinjean, Bazin, Beckers, Deckers, Heremans, Milstein: "Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats." in: European journal of biochemistry / FEBS, Vol. 31, Issue 2, pp. 354-9, 1973 (PubMed).