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  • 產(chǎn)品名稱：Mouseanti-Rat(Rattus)IgE(Chainepsilon)Antibody

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  • 產(chǎn)品廠商：Acris-antibodies
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Mouseanti-Rat(Rattus)IgE(Chainepsilon)Antibody
詳情介紹：
Immunogen IR162, IR1016, IR2 and IR410 rat IgE myeloma proteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone MARE-1
Isotype IgG1
Purification Affinity chromatography on IR2 IgE rat myeloma
Background Synonyms: Rat Immunoglobulin E
Research Area Immunology, Secondary Antibodies
Application Notes ELISA: 5?μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013). Western Blot.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013).
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS,?pH 7.4 containing 0.09?% Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum." in: Journal of immunological methods, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).

Bazin, Xhurdebise, Burtonboy, Lebacq, De Clercq, Cormont: "Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants." in: Journal of immunological methods, Vol. 66, Issue 2, pp. 261-9, 1984 (PubMed).

Bazin, Beckers, Urbain-Vansanten, Pauwels, Bruyns, Tilkin, Platteau, Urbain: "Transplantable IgD immunoglobulin-secreting tumors in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 121, Issue 5, pp. 2077-82, 1979 (PubMed).