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  • 產(chǎn)品名稱：Rabbitanti-HumanIgG(FcRegion)Antibody(Biotin)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Rabbitanti-HumanIgG(FcRegion)Antibody(Biotin)
詳情介紹：
Immunogen Human IgG F(c) fragment.
Characteristics Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Purification Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Note: This product has been assayed against 1.0?μg of Human IgG in a standard captureELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes atroom temperature. A working dilution of 1/10,000-1/100,000 of the reconstitution concentration is suggestedfor this product. Recommended Dilutions: ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride,?pH 7.2 containing 0.01?% (w/v) Sodium Azide as preservative and 10?mg/mL BSA (IgG and Protease free) as stabilizer.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).