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  • 產(chǎn)品名稱:Ratanti-Mouse(Murine)IgM(Chainmu)Antibody(FITC)

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簡單介紹:
Ratanti-Mouse(Murine)IgM(Chainmu)Antibody(FITC)
詳情介紹:
Immunogen Polyclonal and monoclonal mouse IgM. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Clone LO-MM-9
Isotype IgG2a
Purification Affinity chromatography on mouse IgM/Sepharose 4B
Background Synonyms: Immunoglobulin M mouse
Research Area Secondary Antibodies, Immunology
Application Notes Flow Cytometry: 5?μg/mL, use 5 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS,?pH 7.2 containing 0.09?% Sodium Azide and 50?% Glycerol
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum." in: Journal of immunological methods, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).

Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed).