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  • 產(chǎn)品名稱：Mouseanti-HumanIg(LightChain),(Chainlambda)Antibody(FITC)

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Mouseanti-HumanIg(LightChain),(Chainlambda)Antibody(FITC)
詳情介紹：
Immunogen Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Clone Mc24-IC6
Isotype IgG1
Purification Affinity chromatography on Protein A
Alternative Name Immunoglobulin
Background All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5?kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2?mg/L, Lambda, 0.4-4.2?mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Research Area Secondary Antibodies, Virology
Application Notes Flow cytometry: use neat antibody to label 10^6 cells or 10 μL washed whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS,?pH 7.4 containing 0.09?% Sodium Azide and 1?% Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Reynolds, Williamson, Smith, Lane: "A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 123-9, 1992 (PubMed).