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  • 產(chǎn)品名稱：anti-M13BacteriophageG8PCapsidantibody(Biotin)

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anti-M13BacteriophageG8PCapsidantibody(Biotin)
詳情介紹：
Immunogen fd phages from E. coli F+ strain (JM109)
Clone B62-FE2
Isotype IgG2b
Specificity B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (cf. Kneissel et al.)
Purification Affinity Chromatography on Protein A
Background M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.Synonyms: Coat protein B, Gene 8 protein, Major coat protein
Gene ID 927333
NCBI Accession NP_510890
UniProt P69541
Application Notes Phage Display: Immunoassays for the identification of recombinant antigen- orantibody-phages. Detection limit: 10e7 phage particles. Working dilution for ELISA: 1/5,000-1/10,000. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS,?pH 7.4, 0.09?% Sodium Azide, 0.5?% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C up to one month or (in aliquots) at -20 °C for longer.
Background publications Rondot, Koch, Breitling, Dübel: "A helper phage to improve single-chain antibody presentation in phage display." in: Nature biotechnology, Vol. 19, Issue 1, pp. 75-8, 2001 (PubMed).

Kneissel, Queitsch, Petersen, Behrsing, Micheel, Dübel: "Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae)." in: Journal of molecular biology, Vol. 288, Issue 1, pp. 21-8, 1999 (PubMed).

Micheel, Heymann, Scharte, B?ttger, Vogel, Dübel, Breitling, Little, Behrsing: "Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages." in: Journal of immunological methods, Vol. 171, Issue 1, pp. 103-9, 1994 (PubMed).