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  • 產(chǎn)品名稱：IL2 ELISA Kit(Interleukin2)

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IL2 ELISA Kit(Interleukin2)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 2, IL-2 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IL-2.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Interleukin-2,IL-2,T-cell growth factor,TCGF,Il2,Il-2
Alternative Name Il2 (IL2 ELISA Kit Abstract)
Background Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.IL-2 stimulates the proliferation of thymocytes, stimulates the proliferation and differentiation of activated B cells, promotes the growth, differentiation and cytocidal activity of monocytes, induces the growth of and cytokine production and cytolytic activity by natural killer cells, enhances the production of lymphocyte-activated killer (LAK) cells, and induces the proliferation and differentiation of oligodendrocytes . Interleukin 2,IL-2 is a protein of 133 amino acids (15.4 kDa). It is produced mainly by T-cells expressing the surface antigen CD4 following cell activation by mitogens or allogens under physiological conditions. IL-2 displays significant anti-tumor activity for a variety of tumor cell types since it supports the proliferation and clonal expansion of T-cells that specifically attack certain tumor types.
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.