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  • 產(chǎn)品名稱：IL-10 ELISA Kit(Interleukin10)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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IL-10 ELISA Kit(Interleukin10)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 10,IL-10 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IL-10.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10
Alternative Name Il10 (IL10 ELISA Kit Abstract)
Background Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. All known IL-10 family members are secreted -helical proteins. IL-10 is a secreted, possibly glycosylated, polypeptide with a molecular weight of 18 kDa. Based on human studies, IL-10 is likely to circulate as a nondisulfide-linked homodimer. IL-10 is synthesized as a 181 amino acid (aa) precursor with a 19 aa signal sequence and a 162 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which likely form two intra-chain disulfide bridges. Mature rat IL-10 shows 76%, 73%, 72%, 80%, 86%, 85% and 76% aa identity to guinea pig, mouse, human, feline and equine IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells, cytotoxic CD8+ T cells secreting Th2-like cytokines, CD4+CD45RA- (memory) Th1 and Th2 cells, macrophages, monocytes, CD5+ and CD5- B cells, dendritic cells, hepatic stellate (Ito) cells, keratinocytes, melanoma cells, mast cells, placental cytotrophoblasts, and fetal erythroblasts.
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.