產(chǎn)品詳情

  • 產(chǎn)品名稱：AktNegativeControlCellLysate

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Biovision
  • 產(chǎn)品文檔：
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簡單介紹：
AktNegativeControlCellLysate
詳情介紹：
Alternative Name Akt
Background Jurkat cells growing in DMEM supplemented with 10 % FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three volume of Kinase Extraction Buffer (70002-N80M in ABIN411782), keep on ice for 5 min, centrifuge at top speed for 15 min. Collect the cell lysate. Extract the pellet again with one volume Kinase Extraction Buffer. Combine the cell lysate together, determine protein concentration. The cell lysate can be used as a negative control for Akt activity assays, in conjunction with the use of Akt Activity Assay Kit (ABIN411782). We recommend using 100 μg per assay.
Restrictions For Research Use only
Concentration 4 mg/mL
Storage -80 °C
Expiry Date 6 months