產(chǎn)品詳情

  • 產(chǎn)品名稱：SDS-PAGESampleLoadingBuffer(5X)

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  • 產(chǎn)品廠商：Biospes
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SDS-PAGESampleLoadingBuffer(5X)
詳情介紹：
Characteristics SDS-PAGE Sample Loading Buffer is a kind of 5 × concentrated solution which based on Bromophenol blue dye. This reagent contains SDS, DTT, Glycerol, Bromophenol blue, Tris-HCL buffer, ect. It can be used for common SDS－PAGE protein sample loading. Kit Components Components
Sample Preparation
  1. Dissolve the SDS-PAGE Sample Loading Buffer, 5 × in water bath at room temperature or not exceeding 37℃. After dissolving, store at room temperature immediately. 2. Mix sample with SDS-PAGE Sample Loading Buffer, 5 × at the ratio 4:1 (i.e. add 4myl sample into 1myl of SDS-PAGE Sample Loading Buffer). 3. Fully denaturize sample at 100℃ or boiling water bath for 3-5 min. 4. Cool the sample to room temperature, then load and electrophorese. 5. Stop electrophoresis when Bromophenol blue dye arrives at the bottom of gels.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C for 3 months, and at -20 °C for long term.