產品詳情

  • 產品名稱：BECN1293CellLysate

  • 產品型號：
  • 產品廠商：Abbiotec
  • 產品文檔：
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簡單介紹：
BECN1293CellLysate
詳情介紹：
Characteristics Antigen standard for beclin 1, autophagy related (BECN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201629, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name BECN1
Background Synonyms: beclin 1, ATG6 autophagy related 6 homolog, coiled-coil myosin-like BCL2-interacting protein, BECN1, ATG6, beclin1, VPS30
NCBI Accession NP_003757
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for BECN1 293 Cell Lysate (ABIN1595278) BECN1 lysate validation: The BECN1 293 Cell Lysate (lane B) and Control Vector Cell L...